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Features
Clean staining no autofluorescence
Increased signal to noise ratio
User friendly readyto-use format
Compatible with commercial fluor
MedaFluo Non-biotin IF Detection Kits & Autofluorescence Blocking Kit.pdf
Autofluorescence, caused from endogenous fluorophores such as porphyrins, lipofuscin, NADPH, flavins, collagen, elastin, tryptophan, tyrosine, and phenylalanine, Etc., is an intrinsic property of cells and tissues. It can also be caused by the fixatives used. Autofluorescence complicates the use of fluorescence microscopy and interferes with the detection of specific fluorescent signals.
Medaysis ready-to-use Autofluorescence Blocking Kit will reduce or eliminate autofluorescence and increase the signal-to-noise ratio. With the Autofluorescence Blocking Reagent, researchers can clearly visualize and quantify the target signal without interference from the autofluorescence.
Cat. No.: MB001L (100ml) and MB001M (18ml)
Features
Non-biotin signal amplification
Cleaner staining without background
Brighter fluorescence signal
Improved photo- stability
Easy to follow protocol
MedaFluo™ Mouse on Mouse Immunofluorescence Detection
MedaFluo™ Mouse on Human, Rabbit, Goat Immunofluorescence Detection
MedaFluo™ Rabbit on Human, Rabbit, Goat Immunofluorescence Detection
Non-biotin IF Detection Kits
Mouse, Rabbit, Rat Antibodies on Human, Mouse and Rat Tissues or Cell Smears
Medaysis MedaFluo™ immunofluorescence detection kits are specially designed for immunofluorescence detection on formalin-fixed paraffin embedded tissues, cryostat sections, and cell smears. The detection components have gone through extensive absorption to reduce cross reactivity. The kits are utilizing biotin-free signal amplification technologies that eliminate endogenous biotin background to achieve optimal signal-tonoise ratio. MF utilize different conjugating technology thus may be used for multiplexing.
Medaysis provides bright and stable Fluor dyes in all detection systems. Fluor dyes exhibit bright fluorescence intensity and photo stability. Also it remains highly fluorescent over a broad pH range (pH 4-9). These make it an ideal choice for researchers' immunofluorescence applications.
Features
Simultaneous detection of two antigens
Non-biotin signal amplification
Cleaner staining without background
Brighter & stable fluorescence signal
Improved photostability
Easy to follow protocol with RTU reagents in dropper bottles
Mouse, Rabbit, Rat Antibodies on Human, Mouse and Rat Tissues or Cell Smears
Medaysis Duo Fluo™ Immunofluorescence Double Staining Detection Kits are designed to simultaneously visualize two primary antibody-antigen complexes on the same tissue section. Autofluorescence Blocking Reagents are included in the kits to educe/eliminate the autofluorescence on tissue sections to achieve a superior signal/noise ratio. A mounting medium that contains DAPI is included, and it will produce clear and shiny blue nuclear counterstaining.
The Fluor Dyes exhibit bright fluorescence intensity and photostability. Also, it remains highly fluorescent over a broad pH range (pH 4-9). These make it an ideal choice for researchers’ immunofluorescence applications. It uses proprietary fluorescence signal enhancing reagents which also contain agents to reduce non-specific background.
Ultra-Sensitive Mutation Detection Kits Novel and Proprietary Mutation Enrichment Technology
Medaysis’ Ultra-Sensitive Mutation Detection Kits provide Clonal Differential Amplifier (CloDiA™) PCR using novel and proprietary primers to amplify somatic Mutations and suppress or block the amplification of wild-type genes in human genomic DNA.
CloDiA™ PCR includes two types of technique - Unindel™ PCR and Stuntmer™ PCR. Unindel™ PCR is designed to detect a broad range of insertions/deletions (universal insertions/deletions) in the target region. The three-primer set comprising forward primer, reverse primer, and blocker inhibits amplification of wild-type genes but enables amplification of exonic insertions/ deletions.
Stuntmer™ PCR is designed to detect a broad range of Point Mutations in the target region. Dual-port primer of R Port and E Port is designed for self-competition to preferably amply mutated genes but suppress wild-type gene amplification.
This sensitive, specific method enables the detection of less than 1% (as little as 20 ng to 100 ng) of) mutant genes mixed with the wild type. Besides known Mutations, this method can detect unknown Mutations in the target region.
Features and Benefits
Minimal Sample
Small amount of DNA required (20 to 100 ng)
Compatible Samples
FFPE tissues; Fine needle biopsies; Pleural effusion specimens
Broad range of Mutations
Hotspot Mutation analysis of target gene
Ultrasensitive
Detect 0.1-1% mutant genes
Highly Specific
No risk of mispriming; reagents do not encode for the Mutation of interest
Open System
Compatible with various PCR instruments
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