Ultra-Sensitive PTEN mutation detection kit_R233

Price range: $380.00 through $606.00

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Ultra-Sensitive PTEN mutation detection kit is a PCR-based test to enrich somatic mutations of PTEN gene in DNA. With direct sanger sequencing, this kit is able to detect somatic mutation from DNA derived from formalin-fixed paraffin-embedded (FFPET), fine needle biopsy or pleural effusion specimens.

Gene Name
PTEN Phosphatase and tensin homolog

Alternative Name
PTEN, 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, TEP1, phosphatase and tensin homolog, Phosphatase and tensin homolog

GeneID
5728

Uniprot ID
P60484

Target Codon
R233

Covered Gene Range
PTEN Exon 7 Primer Set: 224~267 aa

Lists of components
1. PTEN Exon 7 Primer Set
2. PTEN Exon 7 Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water

Recommend PCR Condition
95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞

Platform
PCR + Sanger sequencing assay

Shipping Condition
4°C

Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles

Intended Use
Research Use Only

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