Ultra-Sensitive KIT mutation detection kit_V560,L576,K642
$454.00 – $849.00
Ultra-Sensitive KIT mutation detection kit is a PCR-based test to enrich somatic mutations of KIT gene in DNA. With direct sanger sequencing, this kit is able to detect 1% somatic mutation from DNA derived from formalin-fixed paraffin-embedded (FFPET), fine needle biopsy or pleural effusion specimens.
Cancer Type
lung, ovary cancer, Leukemia
Intended Use
Research Use Only
Gene Name
KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Official Symbol
KIT
Alternative Name
KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; PBT; C-Kit
GeneID
3815
Uniprot ID
P10721
Target Codon
KIT Exon 11: V560/ L576
KIT Exon 13: K642
Other Interested Codons
KIT Exon 11: K550/ K558/ V559/ N564/ G565/ N567/ Y568/ A569/ T574
KIT Exon 13: V643/ Y646/ L647/ M651
Covered Gene Range
KIT Exon 11(A): 551~591 aa
KIT Exon 11(B): 549~584 aa
KIT Exon 13: 635~663 aa
Lists of components
1. KIT Exon 13 Primer Set
2. KIT Exon 13 Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water
Recommend PCR Condition
95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles.