Ultra-Sensitive ABL mutation detection kit_M244,Q252,Y253,E255


SKU: N/A Category:

Ultra-Sensitive ABL mutation detection kit is a PCR-based test to enrich somatic mutations of ABL gene in DNA. With direct sanger sequencing, this kit is able to detect 1% somatic mutation from DNA derived from formalin-fixed paraffin-embedded (FFPET), fine needle biopsy or pleural effusion specimens.

Cancer Type

Intended Use
Research Use Only

Gene Name
ABL Abelson murine leukemia viral oncogene homolog

Official Symbol

Alternative Name
ABL; ABL1; JTK7; Proto-oncogene tyrosine-protein kinase ABL1; c-ABL; p150; c-abl oncogene 1, non-receptor tyrosine kinase


Uniprot ID

Target Codon
ABL Exon 4: M244/ Q252 / Y253 / E255
ABL Exon 6: T315/ M351

Other Interested Codons
ABL Exon 4: G250
ABL Exon 6: F317/ F359

Covered Gene Range
ABL Exon 4(A): 237~275 aa
ABL Exon 4(B): 184~260 aa
ABL Exon 6(A): 308~361 aa
ABL Exon 6(B): 310~359 aa

Lists of components
1. ABL Exon 4 Primer Set (A)
2. ABL Exon 4 Primer Set (B)
3. ABL Exon 6 Primer Set (A)
4. ABL Exon 6 Primer Set (B)
5. ABL Exon 4 Sequencing Primer-R(A)
6. ABL Exon 4 Sequencing Primer-R(B)
7. ABL Exon 6 Sequencing Primer-R(A)
8. ABL Exon 6 Sequencing Primer-R(B)
9. DNA Quality Control Primer Mix
10. 2x PCR Master Mix
11. Nuclease-Free Water

Recommend PCR Condition
95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞

PCR + Sanger sequencing assay

Shipping Condition

Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles.

Download Datasheet

Download SDS