Ultra-Sensitive ABL mutation detection kit_M244,Q252,Y253,E255
$910.00 – $1,699.00
Ultra-Sensitive ABL mutation detection kit is a PCR-based test to enrich somatic mutations of ABL gene in DNA. With direct sanger sequencing, this kit is able to detect 1% somatic mutation from DNA derived from formalin-fixed paraffin-embedded (FFPET), fine needle biopsy or pleural effusion specimens.
Cancer Type
Leukemia
Intended Use
Research Use Only
Gene Name
ABL Abelson murine leukemia viral oncogene homolog
Official Symbol
ABL
Alternative Name
ABL; ABL1; JTK7; Proto-oncogene tyrosine-protein kinase ABL1; c-ABL; p150; c-abl oncogene 1, non-receptor tyrosine kinase
GeneID
25
Uniprot ID
P00519
Target Codon
ABL Exon 4: M244/ Q252 / Y253 / E255
ABL Exon 6: T315/ M351
Other Interested Codons
ABL Exon 4: G250
ABL Exon 6: F317/ F359
Covered Gene Range
ABL Exon 4(A): 237~275 aa
ABL Exon 4(B): 184~260 aa
ABL Exon 6(A): 308~361 aa
ABL Exon 6(B): 310~359 aa
Lists of components
1. ABL Exon 4 Primer Set (A)
2. ABL Exon 4 Primer Set (B)
3. ABL Exon 6 Primer Set (A)
4. ABL Exon 6 Primer Set (B)
5. ABL Exon 4 Sequencing Primer-R(A)
6. ABL Exon 4 Sequencing Primer-R(B)
7. ABL Exon 6 Sequencing Primer-R(A)
8. ABL Exon 6 Sequencing Primer-R(B)
9. DNA Quality Control Primer Mix
10. 2x PCR Master Mix
11. Nuclease-Free Water
Recommend PCR Condition
95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles.