Smoothelin is a constituent of the smooth muscle cell cytoskeleton protein, exclusively found in differentiated smooth muscle cells (SMC). Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Distinguishing between bladder muscularis mucosae (MM) and muscularis propria (MP) muscle bundles is crucial for accurate staging of bladder carcinoma. Strong smoothelin expression is nearly exclusively observed in muscularis propria. The staining pattern of MP (strongly positive) and MM (negative or weakly positive) makes this technique an attractive diagnostic tool for the sometimes difficult task of staging bladder urothelial carcinoma, such as in transurethral resection specimens of urinary bladder tumors. Differentiating between smooth muscle tumors and other mesenchymal neoplasms of the GI tract can be challenging in small biopsies. Anti-smoothelin immunostaining can be helpful in differentiating benign (+) from malignant smooth muscle tumors (-), and other mimics(-).
Catalog No. MC0471, MC0471RTU7
Clone
4RA
Isotype
IgG1k
Host species
Mouse
Species Reactivity
Human, mouse, rat, chicken
Cellular Localization
cytoplasm
Positive Control
bladder, leiomyoma
Applications
ICC/IF, IHC, IP, WB
Intended Use
Research Use Only