PRC1 Polyclonal

Protein regulator of cytokinesis 1 (PRC1) is a microtubule-bundling protein that is involved in cytokinesis. PRC1 has been identified as a substrate for several Cdks, including Cdc2 and Cdk2. PRC1 binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis. The yeast homolog of PRC1, Ase1, is essential for spindle assembly, elongation and disassembly during mitosis. Ase1 has been shown to undergo degradation mediated by the APC (anaphase-promoting complex) upon entry into G1 phase. Depletion of PRC1 leads to failed cytokinesis. PRC1 is a substrate of CDK1, and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively.