Non-metastatic protein 23 homolog 1; also NDKA or NM23-H1 is a 19‑20 kDa member of the NDK family of enzymes. NM23-H1 is ubiquitous in expression and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NM23-H2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows His and Ser/Thr protein kinase activity and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly and in blood at ng/mL concentrations. Human NM23-H1 is 152 amino acids (aa) in length, contains one NDP kinase domain (aa 5‑134), and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122, and Ser125. Human NM23-H1 shares 89% aa identity with human 17‑18 kDa NM23-H2. The NM23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells.
Catalog No. MC0264, MC0264RTU7
Clone
NM301
Isotype
IgG1k
Host species
Mouse
Species Reactivity
Human, mouse, rat
Cellular Localization
membrane and/or cytoplasm
Positive Control
prostate cancer, brain whole cell lysate; HeLa cells
Applications
IF, IHC, IP
Intended Use
Research Use Only