Interference by autofluorescence is one of the major concerns of immunofluorescence analysis. Autofluorescence, arising from endogenous fluorphore such as porphyrins, lipofuscin, NADPH, flavins, collagen, elastin, tryptophan, tyrosine and phenylalanine etc., is an intrinsic property of cells and tissues. Autofluorescence may also be caused by the fixatives used. It will interfere with detection of specific fluorescent signals, especially when the signals of interest are weak — it causes structures other than those of interest to become visible. Autofluorescence relates to both the specific types of tissues and to the tissue processing procedures. It is important for users to determine if there is any unwanted fluorescence due to either autofluorescence or nonspecific binding of fluorescent label in immunofluorescence application.
Intended Use
Research Use Only. Medaysis MedaFluo™ Autofluorescence Blocking Reagent Kit is designed specifically to reduce or eliminate autofluorescence background on paraffin-embedded tissue section and frozen tissue sections in immunofluorescence procedures without adversely affecting specific fluorescence signals.