MedaFluo™ Immunofluorescence Rat on Mouse Detection Kit is designed to localize rat primary antibody on mouse tissues in immunofluorescence application. When using rat primary antibody on mouse tissue, it becomes hard to choose a satisfactory detection system to distinguish between endogenous mouse immunoglobulins and rat primary antibody since these two species are closely related. The similarity of mouse endogenous immunoglobulins with rat primary antibody will result in high background staining while the specific staining is obscured. To overcome this problem, MedaFluo™ Immunofluorescence Rat on Mouse Detection System has been developed using specially designed and mouse serum absorbed secondary antibody to minimize cross-reactivity to mouse tissues and achieve superior sensitivity. This detection kit utilizes biotin-free signal amplification technology to eliminate endogenous biotin background. Fluor Dyes exhibit bright fluorescence intensity and photostability. Using this kit, researchers may expect the best signal-to-noise ratio comparing to other kits on the market.
Dye
Fluor 594
Excitation/Emission
593/618
Host Species
Rat
Species Reactivity
Mouse
Intended Use
Research Use Only. Medaysis’ MedaFluo™ Immunofluorescence Rat on Mouse Detection Kit (Fluor 594) is designed specifically to localize rat primary antibodies on mouse tissues for immunofluorescence applications. The tissue samples can be paraffin-embedded tissue section, frozen sections or cell smear preparations. This detection kit containing Fluor 594 conjugates will produce a red fluorescence color with excitation wavelength of 593 nm and emission wavelength around 618 nm at the site of rat primary antibodies.