MedaFluo™ Immunofluorescence Mouse on Rat Detection Kit is designed to localize mouse primary antibody on rat tissues in immunofluorescence application. When using mouse primary antibody on rat tissue, it becomes hard to choose a satisfactory detection system to distinguish between endogenous rat immunoglobulins and mouse primary antibody since these two species are closely related. The similarity of rat endogenous immunoglobulins with mouse primary antibody will result in high background staining while the specific staining is obscured. To overcome this problem, MedaFluo™ Immunofluorescence Mouse on Rat Detection System has been developed using specially designed and rat serum absorbed secondary antibody to minimize cross-reactivity to rat tissues and achieve superior sensitivity. This detection kit utilizes biotin-free signal amplification technology to eliminate endogenous biotin background. Fluor Dyes exhibit bright fluorescence intensity and photostability. Using this kit, researchers may expect the best signal-to-noise ratio comparing to other kits on the market.
Dye
Fluor 550
Excitation/Emission
562/576
Host Species
Mouse
Species Reactivity
Rat
Intended Use
Research Use Only. Medaysis’ MedaFluo™ Immunofluorescence Mouse on Rat Detection Kit (Fluor 550) is designed specifically to localize mouse primary antibodies on rat tissues for immunofluorescence applications. The tissue samples can be paraffin-embedded tissue section, frozen sections or cell smear preparations. This detection kit containing Fluor 550 conjugates will produce a yellow fluorescence color with excitation wavelength of 562 nm and emission wavelength around 576 nm at the site of mouse primary antibodies.