MedaFluo™ Non-Biotin Mouse on Mouse Fluorescence Detection Kits is designed to localize mouse primary antibodies on mouse tissue for immunofluorescence applications. The major problem encountered by investigators when they try to stain mouse primary antibodies on mouse tissue using indirect methods is the inability of anti-mouse secondary antibody to distinguish between endogenous mouse immunoglobulin and mouse primary antibody. This problem will cause high background staining while the specific staining is then obscured. Blocking Reagent is specifically designed and formulated to block endogenous immunoglobulin which leads to the most complete elimination of background staining. Together with the proprietary fluorescence signal enhancer which contains background reducing agents, the user may expect the best signal-to-noise ratio using this detection kit.
Dye
Fluor 488
Excitation/Emission
493/518
Host Species
Mouse
Species Reactivity
Mouse
Intended Use
Research Use Only. Medaysis MedaFluo™ Non-Biotin Mouse on Mouse Immunofluorescence Detection Kit (Fluor 488) is designed specifically to localize mouse primary antibodies on mouse tissues for immunofluorescence applications. The tissue samples can be paraffin-embedded mouse tissue section, frozen sections or cell smear preparations. This detection kit containing Fluor 488 conjugates will produce a green fluorescence color with excitation wavelength of 493 nm and emission wavelength around 518 nm at the site of mouse primary antibodies.