MedaFluo™ immunofluorescence mouse detection kit is designed to localize mouse primary antibodies on human, goat and rabbit tissue in immunofluorescence applications. It uses biotin-free fluorescence signal amplification technology and provides superior sensitivity and specificity. This biotin-free technology completely eliminates the potential background due to endogenous biotin activity on biotin-rich tissues when use Biotin-Streptavidin-Fluorophore technology. The Fluor Dyes exhibit bright fluorescence intensity and photostability. Also it remains highly fluorescent over a broad pH range (pH 4-9). These make it an ideal choice for researcher’s immunofluorescence applications. It uses a proprietary fluorescence signal enhancing reagent which also contains agents to reduce non-specific background reducing. Researchers may expect much better signal-to-noise ratio using MedaFluo™ ImmunoFluorescence Detection Kit when compare with the direct labeling technology or other indirect methods, such as Biotin-Streptavidin.
Dye
Fluor 488
Excitation/Emission
493/518
Host Species
Mouse
Species Reactivity
Human, Rabbit, Goat
Intended Use
Research Use Only. Medaysis MedaFluo™ Non-Biotin Mouse on Mouse Immunofluorescence Detection Kit (Fluor 488) is designed specifically to localize mouse primary antibodies on mouse tissues for immunofluorescence applications. The tissue samples can be paraffin-embedded mouse tissue section, frozen sections or cell smear preparations. This detection kit containing Fluor 488 conjugates will produce a green fluorescence color with excitation wavelength of 493 nm and emission wavelength around 518 nm at the site of mouse primary antibodies.