Histone H3 Phospho (PHH3) (pSer10) [MD111R]

Phosphohistone-H3 (PHH3) is a core histone protein, which together with other histones forms the major protein constituents of the chromatin in eukaryotic cells. In mammalian cells, phosphohistone H3 is negligible during interphase but reaches a maximum for chromatin condensation during mitosis. Immunohistochemical studies showed anti-PHH3 detected specifically the core protein histone H3 only when phosphorylated at serine 10. Studies have also revealed no hosphorylation on the histone H3 during apoptosis. Therefore, PHH3 can serve as a mitotic marker to separate mitotic figures from apoptotic bodies and karyorrhectic debris, which may be a very useful tool in diagnosis of tumor grades, especially in CNS, skin, Gyn., Soft tissue, and GIST.