DuoFluo™ 488+650 Rabbit+Rat on Mouse IF Double Staining Detection Kit

$901.00

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DuoFluo™ Rat+Rabbit IF double staining kits are designed to simultaneously localize rat and rabbit primary antibodies cocktail on mouse tissue in IF application. The kits allow simultaneous incubation of primary antibodies and secondary antibodies, totally six steps to stain mouse and rabbit primary antibodies on the same section. The kits utilize proprietary fluorescence signal amplification technology which contains no biotin. It provides superior sensitivity and specificity. The biotin-free technology completely eliminates the potential background due to endogenous biotin activity on biotin-rich tissues when use Biotin-Streptavidin-Fluorophore technology. The Fluor Dyes exhibit bright fluorescence intensity and photostability. Also Fluor dyes will remain highly fluorescent over a broad pH range (pH 4-9). These make it an ideal choice for researcher’s IF application. The proprietary fluorescence signal amplifying reagents also contain agents to reduce non-specific background. Autofluorescence blocking reagents are included in the kits to reduce/eliminate autofluorescence, which will interfere with detection of specific fluorescent signals, especially when the signals of interest are weak – it causes structures other than those of interest to become visible. The autofluorescence blocking reagent will reduce/eliminate autofluorescence without adversely affecting specific fluorescence signals. Researchers may expect the best signal-to-noise ratio using DuoFluo™ IF staining kit when compare with other methods.

Dye
Fluor 488 + 650

Host Species
Rabbit + Rat

Species Reactivity
Mouse

Intended Use
Research Use Only. Medaysis’ DuoFluo™ Rat+Rabbit IF Double Staining Kit (Fluor 488+650, Rat+Rabbit antibodies on Mouse tissue) is designed to simultaneously localize rat and rabbit primary antibody cocktail on mouse tissue. The tissue samples can be paraffin-embedded tissue sections, frozen sections or cell smear preparations. This detection kit containing Fluor 488 conjugates will produce a green fluorescence color with excitation wavelength of 493nm and emission wavelength around 518nm at the site of rat primary antibodies. And it contains Fluor 650 labeled conjugates and it will simultaneously produce a far-red fluorescence color with excitation wavelength of 652nm and emission wavelength around 672nm at the site of rabbit primary antibodies. It is an excellent combination for double staining of co-localization expressions.

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