DuoFluo™ 488+650 Rabbit+Rat on Mouse IF Double Staining Detection Kit

DuoFluo™ Rat+Rabbit IF double staining kits are designed to simultaneously localize rat and rabbit primary antibodies cocktail on mouse tissue in IF application. The kits allow simultaneous incubation of primary antibodies and secondary antibodies, totally six steps to stain mouse and rabbit primary antibodies on the same section. The kits utilize proprietary fluorescence signal amplification technology which contains no biotin. It provides superior sensitivity and specificity. The biotin-free technology completely eliminates the potential background due to endogenous biotin activity on biotin-rich tissues when use Biotin-Streptavidin-Fluorophore technology. The Fluor Dyes exhibit bright fluorescence intensity and photostability. Also Fluor dyes will remain highly fluorescent over a broad pH range (pH 4-9). These make it an ideal choice for researcher’s IF application. The proprietary fluorescence signal amplifying reagents also contain agents to reduce non-specific background. Autofluorescence blocking reagents are included in the kits to reduce/eliminate autofluorescence, which will interfere with detection of specific fluorescent signals, especially when the signals of interest are weak – it causes structures other than those of interest to become visible. The autofluorescence blocking reagent will reduce/eliminate autofluorescence without adversely affecting specific fluorescence signals. Researchers may expect the best signal-to-noise ratio using DuoFluo™ IF staining kit when compare with other methods.