Ultra-Sensitive PTEN mutation detection kit is a PCR-based test to enrich somatic mutations of PTEN gene in DNA. With direct sanger sequencing, this kit is able to detect somatic mutation from DNA derived from formalin-fixed paraffin-embedded (FFPET), fine needle biopsy or pleural effusion specimens.
Gene Name
PTEN Phosphatase and tensin homolog
Alternative Name
PTEN, 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, TEP1, phosphatase and tensin homolog, Phosphatase and tensin homolog
GeneID
5728
Uniprot ID
P60484
Target Codon
R233
Covered Gene Range
PTEN Exon 7 Primer Set: 224~267 aa
Lists of components
1. PTEN Exon 7 Primer Set
2. PTEN Exon 7 Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water
Recommend PCR Condition
95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Intended Use
Research Use Only
