Ultra-Sensitive DNMT3A mutation detection kit is a PCR-based test to enrich somatic mutations of DNMT3A gene in DNA. With direct sanger sequencing, this kit is able to detect 1% somatic mutation from DNA derived from formalin-fixed paraffin-embedded (FFPET), fine needle biopsy or pleural effusion specimens.
Cancer Type
Leukemia
Intended Use
Research Use Only
Gene Name
DNMT3A DNA methyltransferase 3A
Official Symbol
DNMT3A
Alternative Name
DNMT3A; DNA methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA methyltransferase MmuIIIA
GeneID
13435
Uniprot ID
O88508
Target Codon
–
Covered Gene Range
–
Lists of components
1. DNMT3A Primer Set
2. DNMT3A Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water
Recommend PCR Condition
95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles.