Ultra-Sensitive AKT3 Mutation Detection Kit is a PCR-based test to be to detect somatic mutations of AKT gene in DNA derived from formalin-fixed paraffin-embedded (FFPET), fine needle biopsy, blood or pleural effusion specimens.
Intended Use
Research Use Only
Gene Name
AKT3 v-akt murine thymoma viral oncogene homolog 3
Official Symbol
AKT3
Alternative Name
AKT3; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); RAC-gamma serine/threonine-protein kinase; PKBG; PRKBG; RAC gamma; PKB gamma; RAC-gamma serine/threonine protein kinase; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
GeneID
10000
Uniprot ID
Q9Y243
Target Codon
AKT3 Exon 2: E17
Lists of components
1. AKT Exon 2 Primer Set
2. ABL Exon 2 Sequencing Primer-R(A)
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water
Recommend PCR Condition
95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles.