CD14 is a 55-kDa protein found as a glycosylphosphatidylinositol (GPI)- anchored protein on the surface of monocytes, macrophages, and polymorphonuclear leukocytes, and as a soluble protein in the blood. Its main function is to serve as a receptor for lipopolysaccharide (LPS). Besides its role in endotoxin signaling, it has been proposed that CD14 is involved in the transportation of other lipids, cell-cell interactions during different immune responses, and recognition of apoptotic cells. CD14 is highly expressed on the surface of monocytes/macro- phages and strongly up-regulated during the differentiation of monocytic precursor cells into mature monocytes. Therefore, CD14 has been commonly used as a differentiation marker for monocytes/macrophages. Anti-CD14 also labels Langerhans’ cells and dendritic cells.
Clone
MD85R
Isotype
IgG
Host species
Rabbit
Species Reactivity
Human
Cellular Localization
membrane
Positive Control
tonsil, lymph node, appendix, colon, myeloid leukemia
Applications
IHC, WB
Intended Use
Research Use Only